Data/hora: 04/06/2024 17:33:47

Provedor de dados: | 5
País: | France
Título: | A method to assess algicidal activity of microalgal extracts coupling microalgae produced in stirred closed photobioreactor operating in continuous with pulse amplitude modulated (PAM) fluorometry
Autores: | Menguy, Eva; Dumontet, Vincent; Coulombier, Noémie; Meriot, Vincent; Le Dean, Loic; Barthelemy, Vanille; Jauffrais, Thierry.
Data: | 2020
Ano: | 2020
Palavras-chave: | Bioassay; Cytoxicity; Microalgae; Quantum efficiency; Fv/Fm; Non photochemical quenching; NPQ; Electron transport rate; Phytochemistry; Anti algal.
Resumo: | We describe in the present study a quick and reliable method based on chlorophyll a fluorescence to assess putative algicidal effect of different microalgal extracts. We couple microalgal production under chemostat cultivation mode to continuously produce a given microalgae species (e.g. Dunaliella salina in this study) at a stable physiological state to ease comparison between extracts tested; with a non-destructive method based on chlorophyll a fluorescence. Pulse amplitude modulated (PAM) fluorometry was used to assess over time the effect of different microalgal crude extracts on the efficiency of the photosystem II (PSII) of a tested microalgae (Dunaliella salina). • Microalgal production at stationary phase in stirred closed photobioreactor (PBR) operating in continuous have stable photophysiological parameters, which is a prerequisite to compare the impact of different algicidal compounds. • The combination of both methods, allows to quickly assess the algicidal effect of diverse microalgal (crude) extracts on the PSII efficiency of a tested microalgae. • The method may be used to identify and isolate algicidal molecules affecting algal PSII using a bio-guided isolation protocol.
Tipo: | Text
Idioma: | Inglês

Identificador:

https://archimer.ifremer.fr/doc/00643/75524/76400.pdf

DOI:10.1016/j.mex.2020.101037

https://archimer.ifremer.fr/doc/00643/75524/

Editor: | Elsevier BV
Formato: | application/pdf
Fonte: | Methodsx (22150161) (Elsevier BV), 2020 , Vol. 7 , P. 101037 (8p.)

Fonte:

info:eu-repo/semantics/openAccess

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