Data/hora: 04/06/2024 16:43:39

Provedor de dados: | Institutional Archive of Ifreme (ArchiMer)
País: | France
Título: | Campylobacter armoricus sp. nov., a novel member of the Campylobacter lari group isolated from surface water and stools from humans with enteric infection
Autores: | Boukerb, Amine M.; Penny, Christian; Serghine, Joelle; Walczak, Cécile; Cauchie, Henry-michel; Miller, William G.; Losch, Serge; Ragimbeau, Catherine; Mossong, Joël; Mégraud, Francis; Lehours, Philippe; Bénéjat, Lucie; Gourmelon, Michele.
Data: | 2019-12
Ano: | 2019
Palavras-chave: | Campylobacter; Novel species; Surface water; Coastal catchment; Human gastroenteritis; C. lari group; Whole genorne sequence.
Resumo: | During a study on the prevalence and diversity of members of the genus Campylobacter in a shellfish-harvesting area and its catchment in Brittany, France, six urease-positive isolates of members of the genus Campylobacter were recovered from surface water samples, as well as three isolates from stools of humans displaying enteric infection in the same period. These strains were initially identified as members of the Campylobacter lari group by MALDI-TOF mass spectrometry and placed into a distinct group in the genus Campylobacter, following atpA gene sequence analysis based on whole-genome sequencing data. This taxonomic position was confirmed by phylogenetic analysis of the 16S rRNA, rpoB and hsp60 (groEL) loci, and an analysis of the core genome that provided an improved phylogenetic resolution. The average nucleotide identity between the representative strain CA656T (CCUG 73571T=CIP 111675T) and the type strain of the most closely related species Campylobacter ornithocola WBE38T was 88.5 %. The strains were found to be microaerobic and anaerobic, motile, non-spore-forming, Gram-stain-negative, spiral-shaped bacteria that exhibit catalase, oxidase and urease activities but not nitrate reduction. This study demonstrates clearly that the nine isolates represent a novel species within the C. lari group, for which the name Campylobacter armoricus is proposed. Here, we present phenotypic and morphological features of the nine strains and the description of their genome sequences. The proposed type strain CA656T has a 1.589 Mbp chromosome with a DNA G+C content of 28.5 mol% and encodes 1588 predicted coding sequences, 38 tRNAs, and 3 rRNA operons.
Tipo: | Text
Idioma: | Inglês

Identificador:

https://archimer.ifremer.fr/doc/00591/70346/69558.pdf

DOI:10.1099/ijsem.0.003836

https://archimer.ifremer.fr/doc/00591/70346/

Editor: | Microbiology Society
Formato: | application/pdf
Fonte: | International Journal Of Systematic And Evolutionary Microbiology (1466-5026) (Microbiology Society), 2019-12 , Vol. 69 , N. 12 , P. 3969-3979

Fonte:

info:eu-repo/semantics/openAccess

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