Data/hora: 10/06/2024 10:03:05

Provedor de dados: | Institutional Archive of Ifreme (ArchiMer)
País: | France
Título: | Liaison de l'oestradiol 17 beta dans les hepatocytes de truite Arc-en-ciel et induction de la vitellogenine et de son ARNm specifique
Autores: | Maitre, J; Mercier, L; Dolo, L; Valotaire, Y.
Data: | 1983-12
Ano: | 1983
Palavras-chave: | Salmonidae; Pisces; Proteins; Liver; RNA; Steroids; Biosynthesis; Receptors; Sex hormones; Sexual reproduction.
Resumo: | In the liver of rainbow trout (Salmo gairdnerii ), vitellogenin (Vg) synthesis is influenced by oestradiol (E sub(2)) which is believed to act through the classical mechanism of steroid hormone action. After binding of the hormone to a soluble specific receptor protein, the oestradiol-receptor complex is translocated to the nucleus, where it interacts with DNA and modulates expression of Vg genes, leading to increased synthesis of specific mRNA and Vg. The authors show here: (i) the presence of specific oestrogen receptors in the cytosol of the male trout liver. (ii) The male liver, offering, an ideal experimental control of "zero" background, the authors followed - in the liver of male trouts - the kinetics of induction of Vg mRNA by hybridization with Vg cDNA, after E sub(2) stimulation, and (iii) the apparition of Vg in the serum by using an original rocket immuno-electrophoretic technique.
Tipo: | Text
Idioma: | Francês
Identificador: | http://archimer.ifremer.fr/doc/1983/acte-1230.pdf
Editor: | Actes de colloques. Ifremer. Brest [ACTES COLLOQ. IFREMER.]. 1985
Relação: | http://archimer.ifremer.fr/doc/00000/1230/
Formato: | application/pdf