Data/hora: 02/06/2024 00:40:47

Provedor de dados: | Institutional Archive of Ifreme (ArchiMer)
País: | France
Título: | Stylicins, a new family of antimicrobial peptides from the Pacific blue shrimp Litopenaeus stylirostris
Autores: | Rolland, Jean-luc; Abdelouahab, Mahdia; Dupont, J.; Lefevre, F.; Bachere, Evelyne; Romestand, Bernard.
Data: | 2010-03
Ano: | 2010
Palavras-chave: | Ls-Stylicin 1; Peptide; Shrimp; Antifungal; Agglutination; Vibrio.
Resumo: | The present study reports the characterization of Ls-Stylicin1, a novel antimicrobial peptide from the penaeid shrimp, Litopenoeus stylirostris. The predicted mature peptide of 82 residues is negatively charged (theoretical pl=5.0) and characterized by a proline-rich N-terminal region and a C-terminal region containing 13 cysteine residues. The recombinant Ls-Stylicin1 has been isolated in both monomeric and dimeric forms. Both display strong antifungal activity against Fusarium oxysporum (1.25 mu M &lt; MIC &lt;2.5 mu M), a pathogenic fungus of shrimp, but lower antimicrobial activity against Gram () bacteria, Vibrio sp. (40 mu M &lt; MIC &lt;80 mu M). However, rLs-Stylicin1 is able to agglutinate Vibrio pennaeicidae in vitro in agreement with its potent LPS-binding activity on immobilized LPS of V. penaeicidae (dissociation constant (K-d) of 9.6 x 10(-8) M). This molecule with no evident homology to other hitherto described antimicrobial peptides but identified herein several species of penaeid shrimp is thought to be the first member of a shrimp antimicrobial peptide family, which we termed stylicins. (C) 2009 Elsevier Ltd. All rights reserved.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00003/11387/8094.pdf

DOI:10.1016/j.molimm.2009.12.007

Editor: | Pergamon-elsevier Science Ltd
Relação: | http://archimer.ifremer.fr/doc/00003/11387/
Formato: | application/pdf
Fonte: | Molecular Immunology (0161-5890) (Pergamon-elsevier Science Ltd), 2010-03 , Vol. 47 , N. 6 , P. 1269-1277
Fonte: | 2010 Elsevier Ltd All rights reserved.