Data/hora: 03/06/2024 08:35:25

Provedor de dados: 	Institutional Archive of Ifreme (ArchiMer)	
País: 	France	
Título: 	Transcriptomic analysis of clam extra pallial fluids reveals immunity and cytoskeleton alterations in the first week of Brown Ring Disease development	
Autores: 	Rahmani, Alexandra; Corre, Erwan; Richard, Gaëlle; Bidault, Adeline; Lambert, Christophe; Oliveira, Louisi; Thompson, Cristiane; Thompson, Fabiano; Pichereau, Vianney; Paillard, Christine.	
Data: 	2019-10	
Ano: 	2019	
Palavras-chave: 	Brown ring disease; V. tapetis; R. philippinarum; Hemocytes; Actin cytoskeleton; Beta-Thymosin; Coactosin; Resting cells.	
Resumo: 	The Brown Ring Disease is an infection caused by the bacterium Vibrio tapetis on the Manila clam Ruditapes philippinarum. The process of infection, in the extrapallial fluids (EPFs) of clams, involves alteration of immune functions, in particular on hemocytes which are the cells responsible of phagocytosis. Disorganization of the actin-cytoskeleton in infected clams is a part of what leads to this alteration. This study is the first transcriptomic approach based on collection of extrapallial fluids on living animals experimentally infected by V. tapetis. We performed differential gene expression analysis of EPFs in two experimental treatments (healthy-against infected-clams by V. tapetis), and showed the deregulation of 135 genes. In infected clams, a downregulation of transcripts implied in immune functions (lysosomal activity and complement- and lectin-dependent PRR pathways) was observed during infection. We also showed a deregulation of transcripts encoding proteins involved in the actin cytoskeleton organization such as an overexpression of β12-Thymosin (which is an actin sequestration protein) or a downregulation of proteins that closely interact with capping proteins such as Coactosin, that counteract action of capping proteins, or Profilin. We validated these transcriptomic results by cellular physiological analyses that showed a decrease of the lysosome amounts and the disorganization of actin cytoskeleton in infected hemocytes.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	https://archimer.ifremer.fr/doc/00510/62131/66362.pdf

DOI:10.1016/j.fsi.2019.08.025

https://archimer.ifremer.fr/doc/00510/62131/	
Editor: 	Elsevier BV	
Relação: 	info:eu-repo/grantAgreement/EC/FP7/678589/EU//VIVALDI	
Formato: 	application/pdf	
Fonte: 	Fish &amp; Shellfish Immunology (1050-4648) (Elsevier BV), 2019-10 , Vol. 93 , P. 940-948	
Fonte: 	info:eu-repo/semantics/openAccess

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