Data/hora: 02/06/2024 01:15:25

Provedor de dados: 	Institutional Archive of Ifreme (ArchiMer)	
País: 	France	
Título: 	Entrapment of anaerobic thermophilic and hyperthermophilic marine microorganisms in a gellan/xanthan matrix	
Autores: 	Landreau, M.; Duthoit, Frederique; Claeys-bruno, M.; Vandenabeele-trambouze, O.; Aubry, T.; Godfroy, Anne; Le Blay, Gwenaelle.	
Data: 	2016-06	
Ano: 	2016	
Palavras-chave: 	(hyper)thermophilic marine micro-organisms; Anaerobiosis; Entrapment; Gellan; Immobilization; Xanthan.	
Resumo: 	Aims  The aims of this study were (i) to develop a protocol for the entrapment of anaerobic (hyper)thermophilic marine micro-organisms; (ii) to test the use of the chosen polymers in a range of physical and chemical conditions and (iii) to validate the method with batch cultures. Methods and Results  The best conditions for immobilization were obtained at 80°C with gellan and xanthan gums. After 5-week incubation, beads showed a good resistance to all tested conditions except those simultaneously including high temperature (100°C), low NaCl (&lt;0∙5 mol l−1) and extreme pH (4/8). To confirm the method efficiency, batch cultures with immobilized Thermosipho sp. strain AT1272 and Thermococcus kodakarensis strain KOD1 showed an absence of detrimental effect on cell viability and a good growth within and outside the beads. Conclusion  This suggests that entrapment in a gellan–xanthan matrix could be employed for the culture of anaerobic (hyper)thermophilic marine micro-organisms. Significance and Impact of the Study  (Hyper)thermophilic marine micro-organisms possess a high biotechnological potential. Generally microbial cells are grown as free-cell cultures. The use of immobilized cells may offer several advantages such as protection against phage attack, high cell biomass and better production rate of desired metabolites.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00332/44352/44000.pdf

DOI:10.1111/jam.13118

http://archimer.ifremer.fr/doc/00332/44352/	
Editor: 	Wiley-blackwell	
Relação: 	info:eu-repo/grantAgreement/EC/FP7/311975/EU//MACUMBA	
Formato: 	application/pdf	
Fonte: 	Journal Of Applied Microbiology (1364-5072) (Wiley-blackwell), 2016-06 , Vol. 120 , N. 6 , P. 1531-1541	
Fonte: 	2016 The Society for Applied Microbiology

info:eu-repo/semantics/openAccess

restricted use