Data/hora: 09/06/2024 03:43:54

Provedor de dados: | 5
País: | France
Título: | Entrapment of anaerobic thermophilic and hyperthermophilic marine microorganisms in a gellan/xanthan matrix
Autores: | Landreau, M.; Duthoit, Frederique; Claeys-bruno, M.; Vandenabeele-trambouze, O.; Aubry, T.; Godfroy, Anne; Le Blay, Gwenaelle.
Data: | 2016-06
Ano: | 2016
Palavras-chave: | (hyper)thermophilic marine micro-organisms; Anaerobiosis; Entrapment; Gellan; Immobilization; Xanthan.
Resumo: | Aims  The aims of this study were (i) to develop a protocol for the entrapment of anaerobic (hyper)thermophilic marine micro-organisms; (ii) to test the use of the chosen polymers in a range of physical and chemical conditions and (iii) to validate the method with batch cultures. Methods and Results  The best conditions for immobilization were obtained at 80°C with gellan and xanthan gums. After 5-week incubation, beads showed a good resistance to all tested conditions except those simultaneously including high temperature (100°C), low NaCl (&lt;0∙5 mol l−1) and extreme pH (4/8). To confirm the method efficiency, batch cultures with immobilized Thermosipho sp. strain AT1272 and Thermococcus kodakarensis strain KOD1 showed an absence of detrimental effect on cell viability and a good growth within and outside the beads. Conclusion  This suggests that entrapment in a gellan–xanthan matrix could be employed for the culture of anaerobic (hyper)thermophilic marine micro-organisms. Significance and Impact of the Study  (Hyper)thermophilic marine micro-organisms possess a high biotechnological potential. Generally microbial cells are grown as free-cell cultures. The use of immobilized cells may offer several advantages such as protection against phage attack, high cell biomass and better production rate of desired metabolites.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00332/44352/44000.pdf

DOI:10.1111/jam.13118

http://archimer.ifremer.fr/doc/00332/44352/

Editor: | Wiley-blackwell
Relação: | info:eu-repo/grantAgreement/EC/FP7/311975/EU//MACUMBA
Formato: | application/pdf
Fonte: | Journal Of Applied Microbiology (1364-5072) (Wiley-blackwell), 2016-06 , Vol. 120 , N. 6 , P. 1531-1541

Fonte:

2016 The Society for Applied Microbiology

info:eu-repo/semantics/openAccess

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