Data/hora: 05/06/2024 16:10:23

Provedor de dados: 	5	
País: 	France	
Título: 	Cytodétection des phycotoxines diarrhéiques - Une alternative au test de toxicité aiguë sur souris pour la surveillance de la salubrité des coquillages	
Autores: 	Amzil, Zouher.	
Data: 	1996-09	
Ano: 	1996	
Palavras-chave: 	Acide okadaïque; Inhibition des protéines phosphatases; Cytodétection; Cellules KB; Okadaic acid; Inhibition of protein-phosphatase; Cytodeteciton KB-cells.	
Resumo: 	The cytodetection test of diarrheic phycotoxin KB-cells (DRAME test) was developed to ultimately provide a substitute for the acute toxicity mouse-test currently used by IFREMER's phytoplankton monitoring network (REPHY). The cyctodetection test relies on the cell morphological changes induced by the phosphorylation of cytoskeleton proteins resulting from the inhibition of cell protein-phosphatases by okadaic-type toxins. The method is specific, sensitive and fast with results given on the same day. In addition, the method has been shown to produce a good correlation with the acute toxicity mouse-test as weil as with HPLC analyses of diarrheic phycotoxins. The cytodetection test was awarded the only Special Prize of the Jury at the 6th AMAL THEE Awards organized by OPAL (Oeuvre pour l'Assistance aux Animaux de Laboratoire - Fund for Assistance to Laboratory Animais). The proposed study concerns an experimental trial of this okadaic acid cyctodetection method in contaminated shellfish under real-scale conditions of application.	
Tipo: 	Text	
Idioma: 	Francês	
Identificador: 	http://archimer.ifremer.fr/doc/00156/26730/24811.pdf	
Relação: 	http://archimer.ifremer.fr/doc/00156/26730/	
Formato: 	application/pdf	
Fonte: 	1996 Ifremer