Data/hora: 11/06/2024 14:35:40

Provedor de dados: | Institutional Archive of Ifreme (ArchiMer)
País: | France
Título: | Detection of ostreid herpesvirus 1 DNA by PCR in bivalve molluscs: A critical review
Autores: | Batista, Frederico; Arzul, Isabelle; Pepin, Jean-francois; Ruano, Francisco; Friedman, Carolyn S.; Boudry, Pierre; Renault, Tristan.
Data: | 2007
Ano: | 2007
Palavras-chave: | Oyster; Bivalve molluscs; PCR; Detection; OsHV 1; Herpesvirus.
Resumo: | Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1 variant. The literature on DNA extraction methods, primers, PCR strategies, and confirmatory procedures used for the detection and identification of herpesviruses that infect bivalve molluscs are reviewed. (c) 2006 Elsevier B.V. All rights reserved.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2007/publication-2322.pdf

DOI:10.1016/j.jviromet.2006.09.005

Editor: | Elsevier
Relação: | http://archimer.ifremer.fr/doc/00000/2322/
Formato: | application/pdf
Fonte: | Journal of Virological Methods (0166-0934) (Elsevier), 2007 , Vol. 139 , N. 1 , P. 1-11
Fonte: | 2006 Elsevier