Data/hora: 05/06/2024 08:57:01

Provedor de dados: | 5
País: | France
Título: | A complementary method for production of tetraploid Crassostrea gigas using crosses between diploids and tetraploids with cytochalasin B treatments
Autores: | Mccombie, Helen; Ledu, Christophe; Phelipot, Pascal; Lapegue, Sylvie; Boudry, Pierre; Gerard, Andre.
Data: | 2005-08
Ano: | 2005
Palavras-chave: | Oyster breeding; Gynogenesis; Polar body; Cytochalasin B; Polyploidy; Crassostrea gigas.
Resumo: | We present a new method to produce tetraploid Crassostrea gigas by cytochalasin B inhibition of polar body 2 expulsion in diploid females crossed with tetraploid males. This offers a means of direct introgression of genetic characters from selected diploid to tetraploid lines, avoiding a triploid step. Offspring larval ploidy shifted over time and depended on size, with tetraploids more frequent among the smaller larvae and triploids among the large. Viable tetraploids were found at 4 and 6 months, indicating the technique was successful. The possibility that gynogenesis occurred was tested by microsatellite analysis to confirm the presence of paternally inherited alleles. These were present in all animals of the 2n x 4n + CB (female first) cross. However, a 4n x 2n + CB cross produced triploids, including some gynogens. Our method illustrates for the first time that diploid C. gigas eggs, if selected for large size, can give viable tetraploid offspring.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2005/publication-632.pdf

DOI:10.1007/s10126-004-0440-2

Editor: | Springer
Relação: | http://archimer.ifremer.fr/doc/00000/632/
Formato: | application/pdf
Fonte: | Marine Biotechnology (1436-2228) (Springer), 2005-08 , Vol. 7 , N. 4 , P. 318-330
Fonte: | 2005 Publisher: Springer-Verlag New York