Data/hora: 17/06/2024 06:39:33

Provedor de dados: | 5
País: | France
Título: | The use of fluorescent Nile red and BODIPY for lipid measurement in microalgae
Autores: | Rumin, Judith; Bonnefond, Hubert; Saint-jean, Bruno; Rouxel, Catherine; Sciandra, Antoine; Bernard, Olivier; Cadoret, Jean-paul; Bougaran, Gael.
Data: | 2015-03
Ano: | 2015
Palavras-chave: | Nile red; BODIPY 505/515; Microalgae; Neutral lipid; Fluorescence; Biodiesel.
Resumo: | Microalgae are currently emerging as one of the most promising alternative sources for the next generation of food, feed, cosmetics and renewable energy in the form of biofuel. Microalgae constitute a diverse group of microorganisms with advantages like fast and efficient growth. In addition, they do not compete for arable land and offer very high lipid yield potential. Major challenges for the development of this resource are to select lipid-rich strains using high-throughput staining for neutral lipid content in microalgae species. For this purpose, the fluorescent dyes most commonly used to quantify lipids are Nile red and BODIPY 505/515. Their fluorescent staining for lipids offers a rapid and inexpensive analysis tool to measure neutral lipid content, avoiding time-consuming and costly gravimetric analysis. This review collates and presents recent advances in algal lipid staining and focuses on Nile red and BODIPY 505/515 staining characteristics. The available literature addresses the limitations of fluorescent dyes under certain conditions, such as spectral properties, dye concentrations, cell concentrations, temperature and incubation duration. Moreover, the overall conclusion of the present review study gives limitations on the use of fluorochrome for screening of lipid-rich microalgae species and suggests improved protocols for staining recalcitrant microalgae and recommendations for the staining quantification.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00257/36823/35451.pdf

DOI:10.1186/s13068-015-0220-4

http://archimer.ifremer.fr/doc/00257/36823/

Editor: | Biomed Central Ltd
Formato: | application/pdf
Fonte: | Biotechnology For Biofuels (1754-6834) (Biomed Central Ltd), 2015-03 , Vol. 8 , N. 42 , P. 1-16

Fonte:

2015 Rumin et al.; licensee BioMed Central. This is an OpenAccess article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated

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