Data/hora: 03/06/2024 18:30:43

Provedor de dados: | 5
País: | France
Título: | L'éthoxyrésorufine-o-déethylase, les adduits à l'ADN et les mocronuclei dans les organismes marins. Application à la surveillance des effets biologiques sur les côtes françaises
Autores: | Burgeot, Thierry.
Data: | 1994
Ano: | 1994
Palavras-chave: | Ethoxyrésorufine-o-déethylase; Adduits à l'ADN; Micronuclei; Poisson; Moule; Huître; Biosurveillance; Ethoryresorufin-o-deethylase; DNA adducts; Micronuclei; Fish; Mussel; Oyster; Biomonitoring.
Resumo: | Three biomarkers [ethoxyresorufm-o-deethylase (EROD), DNA adducts and micronuclei (MN) were studied for biomonitoring applications along French coasts. EROD was developed at an experirnental stage in two pilot sites, Seine Bay and Fos sur Mer, using the dab (Limanda limanda), dragon et (Callionymus lyra), red mullet (Mullus barbatus) and comber (Serranus hepatus) as target species. Higbest EROD activity was observed in the subestuarial part of Seine Bay and at the stations closest to sources of pollution from Cortiou and Fos sur Mer. The suitable number of fish for detection of a significant difference between two EROD activities was deterrnined as a function of sampling variance and a preselected statistical power. A preliminary study on hepatocytes in a primary dragonet culture enabled us to evaluate the toxic cffect of PCB in the marine environment. DNA PAH adducts were measured at the same time as EROD in the liver ofred mullet trawled in the Mediterranean, and PAH was analyzed in sediments. Specific DNA PAH adducts were detected in highly polluted areas. The genotoxic effects of MN were measured in vitro and in vivo in the mussel (Mytilus galloprovincialis) and oyster (Crassostrea gigas). Poor sensitivity was found for oysters along a pollution gradient in the Gironde estuary, but greater sensitivity was obtained when heart cells in primary culture were contaminated with benzo[a)pyrene, cupric sulfate and paper mill effluent. Variations in MN frequency were measured in mussels during four months of monitoring at highly contaminated sites. The major drawback of the MN test for monitoring purposes is that it entails laborious and sometimes subjective microscopie observation.
Tipo: | Text
Idioma: | Francês

Identificador:

http://archimer.ifremer.fr/doc/00443/55462/56984.pdf

http://archimer.ifremer.fr/doc/00443/55462/

Editor: | Université de Nantes
Formato: | application/pdf

Fonte:

1994 Université de Nantes

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