Data/hora: 01/06/2024 16:24:54

Provedor de dados: | Institutional Archive of Ifreme (ArchiMer)
País: | France
Título: | Rapid and sensitive PCR detection of Vibrio penaeicida, the putative etiological agent of syndrome 93 in New Caledonia
Autores: | Saulnier, Denis; Avarre, Jean-christophe; Le Moullac, Gilles; Ansquer, Dominique; Levy, Peva; Vonau, Vincent.
Data: | 2000-03
Ano: | 2000
Palavras-chave: | Small subunit rDNA gene; Penaeus (Litopenaeus) stylirostris; Vibriosis; Shrimp disease; PCR diagnosis; Vibrio penaeicida; Syndrome 93.
Resumo: | Experimental infections of Penaeus (Litopenaeus) stylirostris were performed with a Vibrio penaeicida strain (AM101) isolated in New Caledonia from Syndrome 93 diseased shrimp. Cumulative mortalities resulting from intramuscular injection or immersion of shrimp in bacterial suspensions demonstrated high virulence for this bacterial strain and suggested that V. penaeicida could be the etiological agent of Syndrome 93. The median lethal dose (LD50) for AM101 was 1.3 x 104 CFU (colony forming units) ml-1 by immersion and less than 5 CFU shrimp-1 by intramuscular challenge, with mortality outbreaks at 48 and 22 h after challenge, respectively. A polymerase chain reaction (PCR) detection assay using a primer set designed from the 16S ribosomal RNA gene of V. penaeicida was developed. It gave an expected amplicon of approximately 310 bp in ethidium bromide-stained agarose gels. The specificity of these primers was assessed with different Vibrio species. Furthermore, DNA extracted by the ChelexTM method could be used to detect fewer than 20 cultured Vibrio cells in seawater or shrimp hemolymph by this assay. It appears to be a reliable screening method for detecting V. penaeicida in shrimp and from the aquatic environment.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2000/publication-4436.pdf

DOI:10.3354/dao040109

Editor: | Inter-Research
Relação: | http://archimer.ifremer.fr/doc/00000/4436/
Formato: | application/pdf
Fonte: | Diseases of aquatic organisms (0177-5103) (Inter-Research), 2000-03 , Vol. 49 , N. 2 , P. 109-115
Fonte: | Inter-Research 2000