Data/hora: 16/06/2024 11:08:53

Provedor de dados: 	5	
País: 	France	
Título: 	Natural abundance of N-15 and C-13 in fish tissues and the use of stable isotopes as dietary protein tracers in rainbow trout and gilthead sea bream	
Autores: 	Beltran, M.; Fernandez-borras, J.; Medale, Francoise; Perez-sanchez, J.; Kaushik, Sadasivam; Blasco, J.	
Data: 	2009-02	
Ano: 	2009	
Palavras-chave: 	Sea bream; Rainbow trout; Protein; Diet; Delta N 15; Delta C 13.	
Resumo: 	For developing efficient diets, two sets of experiments examined whether the use and allocation of dietary protein can be traced by labelling with stable isotopes (N-15 and C-13) in two culture fish (Oncorhynchus mykiss and Sparus aurata). In the first experiment, natural abundance and tissue distribution of these isotopes were determined, by measuring the delta C-13 and delta N-15 values by isotopic ratio mass spectrometry, in fingerlings (14-17 g) adapted to diets differing in the percentage of fish meal replacement by plant protein sources. For both species, delta N-15 and delta C-13 were greater in tissues with higher protein and lower lipid content. Delta N-15 of diets and tissues decreased as replacement increased, suggesting delta N-15 can be used as a marker for dietary protein origin. The N-15 fractionation (delta N-15 fish - delta N-15 diet) differed between groups, and could thus be used to indicate protein catabolism. In the second experiment, fish (75-90 g) of each species ingested a diet enriched with N-15-protein (10 g kg(-1) diet) and C-13-protein (30 g kg(-1) diet). These proportions were suitable for determining that the delta values of tissue components were high enough above natural levels to allow protein allocation to be traced at 11 and 24 h after feeding, and revealed clear metabolic differences between species.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/2009/publication-6966.pdf

DOI:10.1111/j.1365-2095.2008.00563.x	
Editor: 	Wiley / Blackwell	
Relação: 	http://archimer.ifremer.fr/doc/00000/6966/	
Formato: 	application/pdf	
Fonte: 	Aquaculture Nutrition (1353-5773) (Wiley / Blackwell), 2009-02 , Vol. 15 , N. 1 , P. 9-18	
Fonte: 	2009 Blackwell Publishing