Data/hora: 09/06/2024 05:40:28

Provedor de dados: | Institutional Archive of Ifreme (ArchiMer)
País: | France
Título: | RpoB-PCR amplified gene and temporal temperature gradient gel electrophoresis: a rapid tool to analyse bacterial strains representative of cold-smoked salmon microflora
Autores: | Giacomazzi, Sophie; Leroi, Francoise; L'Henaff, Cecile; Joffraud, Jean-jacques.
Data: | 2004-02
Ano: | 2004
Palavras-chave: | TTGE; RpoB; Nested PCR; Cold smoked salmon flora.
Resumo: | Aims: To evaluate rpoB gene as a biomarker of microbial biodiversity associated to cold-smoked salmon by a novel nested-polymerase chain reaction/temporal temperature gradient gel electrophoresis (PCR/TTGE) technique applied on pure cultures of reference strains.   Methods and Results: DNA obtained from pure cultures of reference strains was used in a succession of a first PCR amplification of rpoB fragment with degenerated nonclamped primers and a nested-PCR with nondegenerated clamped primers. PCR products were then applied on a TTGE gel in order to analyse strains profile. High quantity of nested-PCR products were obtained for each tested strain and TTGE profiles showed a good separation between the different reference bacteria and an easy way to associate one band to one species.   Conclusion: The nested-PCR/TTGE technique used in this study is a promising way of investigating bacterial community structure of cold-smoked salmon or other food matrix.   Significance and Impact of the Study: Because of its single copy state leading to single band profiles in TTGE, rpoB constitute a good potential molecular marker for further development of cold-smoked salmon biodiversity analysis.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2004/publication-579.pdf

DOI:10.1111/j.1472-765X.2003.01463.x

Editor: | Blackwell science
Relação: | http://archimer.ifremer.fr/doc/00000/579/
Formato: | application/pdf
Fonte: | Letters in Applied Microbiology (0266-8254) (Blackwell science), 2004-02 , Vol. 38 , N. 2 , P. 130-134
Fonte: | 2004 Blackwell Synergy