Data/hora: 09/06/2024 04:38:23

Provedor de dados: | 5
País: | France
Título: | Monitoring of four DNA extraction methods upstream of high-throughput sequencing of Anisakidae nematodes
Autores: | Seesao, Y.; Audebert, C.; Verrez-bagnis, Veronique; Merlin, S.; Jerome, Marc; Viscogliosi, E.; Dei-cas, E.; Aliouat-denis, C. M.; Gay, Melanie.
Data: | 2014-07
Ano: | 2014
Palavras-chave: | Anisakidae; High-throughput sequencing; Nematode; Pooled DNA extraction; Marine fish; COX2.
Resumo: | Different methods were evaluated to extract DNA from pooled nematodes belonging to Anisakis, Contracaecum, Pseudoterranova and Hysterothylacium genera isolated from edible fish. Pooled DNA extraction is the first and compulsory step to allow the identification of a large number of samples through high-throughput DNA sequencing with drastic time and cost reductions.
Tipo: | Text
Idioma: | Inglês

Identificador:

https://archimer.ifremer.fr/doc/00189/30020/28607.pdf

DOI:10.1016/j.mimet.2014.05.004

https://archimer.ifremer.fr/doc/00189/30020/

Editor: | Elsevier Science Bv
Formato: | application/pdf
Fonte: | Journal Of Microbiological Methods (0167-7012) (Elsevier Science Bv), 2014-07 , Vol. 102 , P. 69-72

Fonte:

2014 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

info:eu-repo/semantics/openAccess

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