Data/hora: 01/06/2024 07:21:26

Provedor de dados: | Institutional Archive of Ifreme (ArchiMer)
País: | France
Título: | Imagerie par spectrométrie de masse de l’accumulation d’acide okadaïque dans la glande digestive de la moule
Autores: | Sam Lone, Anais.
Data: | 2016-08
Ano: | 2016
Palavras-chave: | Imagerie par spectrométrie de masse; MALDI-TOF; TOF-SIMS; Acide okadaïque; Glande digestive; Mytilus edulis; Imaging by mass spectrometry; MALDI-TOF; TOF-SIMS; Okadaic acid; Digestive Gland; Mytilus edulis.
Resumo: | Among the applications that facilitate the study of functional processes in living systems, measurement of the spatial distribution of molecules in biological tissues is currently available. Thus, in the field of human health, this application which targets the molecular distributions of altered tissues can be used, for example, in the diagnosis of diseases such as cancers, Alzheimer's disease, muscular myopathy, but also to recognize the distribution of drugs in animal tissues. The principle of imaging by mass spectrometry is to produce a mapping of the surface of a section of tissue, recording at each point a mass spectrum. All these spectra reconstitute so many "ion density maps", called images, that there are peaks of biological interest in the spectra. Previous studies published by the Institute of Chemistry of Natural Substances (CNRS UPR2301) have shown the interest of localized analyzes for the study of plaques of atheroma, steatosis and liver cirrhosis, or genetic diseases such as Fabry, Duchenne muscular dystrophy, cystic fibrosis. The study reported here, carried out in collaboration with the Phycotoxins laboratory at Ifremer in Nantes, allowed to deepen tests obtained in a study aiming at detecting and locating the okadaic acid (AO) in mussels and giving promising results (Guitton, Mondeguer et al., 2012). AO is one of the dinophysistoxins produced by dinoflagellates of the genus Prorocentrum and Dinophysis. This diarrheic toxin, predominantly bio-concentrated in mussels digestive glands (Blanco, Morono et al., 2005), is a secondary metabolite synthesized by the microalgae. Studies conducted to date with biochemical tools to locate toxins in a subcellular manner and search for markers related to the presence of toxins have not led to major advances. Concerning the link between the AO and its sequestration site in bivalves, most of the studies carried out on mussels show that a large part of the AO that is easily detachable is related to the digestive cells while a smaller fraction of okadaic acid, perhaps a lipoprotein (Rossignoli and Blanco, 2010), would be strongly related to the secretory cells of the digestive gland. MALDI-TOF mass spectrometry imaging using a Bruker UltrafleXtreme mass spectrometer was the tool used to attempt to detect and quantify okadaic acid on digestive gland of toxic mussel cross-section surface. Changes in the lipid composition were also investigated. TOF-SIMS mass spectrometry imaging was also used to attempt more accurate micron scale locations
Tipo: | Text
Idioma: | Francês

Identificador:

http://archimer.ifremer.fr/doc/00360/47167/47190.pdf

http://archimer.ifremer.fr/doc/00360/47167/

Formato: | application/pdf

Fonte:

2016 CNRS, ICSN, Université Paris-Saclay

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