Data/hora: 04/06/2024 02:47:58

Provedor de dados: | 5
País: | France
Título: | Combining Two-Stage Testing and Interval Mapping Strategies to Detect QTL for Resistance to Bonamiosis in the European Flat Oyster Ostrea edulis
Autores: | Lallias, Delphine; Gomez-raya, L.; Haley, C. S.; Arzul, Isabelle; Heurtebise, Serge; Beaumont, A. R.; Boudry, Pierre; Lapegue, Sylvie.
Data: | 2009-10
Ano: | 2009
Palavras-chave: | Statistical power; Survival analysis; QTL mapping; Disease resistance; Ostrea edulis.
Resumo: | We have identified quantitative trait loci (QTL) in the flat oyster (Ostrea edulis) for resistance to Bonamia ostreae, a parasite responsible for the dramatic reduction in the aquaculture of this species. An F-2 family from a cross between a wild oyster and an individual from a family selected for resistance to bonamiosis was cultured with wild oysters injected with the parasite, leading to 20% cumulative mortality. Selective genotyping of 92 out of a total of 550 F-2 progeny (i.e., 46 heavily infected oysters that died and 46 parasite-free oysters that survived) was performed using 20 microsatellites and 34 amplification fragment length polymorphism primer pairs. Both a two-stage testing strategy and QTL interval mapping methods were used. The two-stage detection strategy had a high power with a low rate of false positives and identified nine and six probable markers linked to genes of resistance and susceptibility, respectively. Parent-specific genetic linkage maps were built for the family, spanning ten linkage groups (n = 10) with an observed genome coverage of 69-84%. Three QTL were identified by interval mapping in the first parental map and two in the second. Good concordance was observed between the results obtained after the two-stage testing strategy and QTL mapping.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2009/publication-6682.pdf

DOI:10.1007/s10126-008-9173-y

Editor: | Springer
Relação: | http://archimer.ifremer.fr/doc/00000/6682/
Formato: | application/pdf
Fonte: | Marine Biotechnology (1436-2228) (Springer), 2009-10 , Vol. 11 , N. 5 , P. 570-584
Fonte: | 2009 Springer