Data/hora: 04/06/2024 07:38:40

Provedor de dados: | 5
País: | France
Título: | Molecular characterisation of prolactin and analysis of extrapituitary expression in the European sea bass Dicentrarchus labrax under various salinity conditions
Autores: | Boutet, Isabelle; Lorin Nebel, C; De Lorgeril, Julien; Guinand, B.
Data: | 2007-03
Ano: | 2007
Palavras-chave: | Prolactin; Phylogenctic analysis; MRNA expression; Osmoregulation; Intestine; Gill; Dicentrarchuslabrax.
Resumo: | dAlthough prolactin has been demonstrated to be the main hormone involved in adaptation to dilute media in several freshwater teleosts, few studies have been conducted in marine teleosts. In the Mediterranean, the sea bass Dicentrarchus labrax inhabits environments ranging from the open sea to coastal lagoons, where salinity varies greatly. We characterised the prolactin (prl) gene and analysed its expression in two organs (gill and intestine) in D. labrax acclimated to either freshwater or seawater. A 2819 bp long sequence encompassing the prl gene and a part (282 bp) of the promoter were identified, and these comprised 5 coding exons separated by 4 introns. Prolactin was similarly expressed in fresh- and seawater adapted fish, although expression in gills was significantly greater than in the intestine. Nonetheless, individuals unable to successfully regulate osmotic balance in freshwater presented overall low expression rates. Results are discussed according to the mechanism of sea bass adaptation in the wild and to their life cycle between open sea and lagoons. Finally, a phylogenetic analysis indicated that teleosts are not branched according to their life-history features (e.g. seawater vs. freshwater habitats), and no signature of positive selection was detected across the phylogeny of the prl gene in teleosts. (c) 2006 Elsevier Inc. All rights reserved.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2007/publication-2537.pdf

DOI:10.1016/j.cbd.2006.12.002

Editor: | Elsevier
Relação: | http://archimer.ifremer.fr/doc/00000/2537/
Formato: | application/pdf
Fonte: | Comparative Biochemistry and Physiology Part D: Genomics and Proteomics (1744-117X) (Elsevier), 2007-03 , Vol. 2 , N. 1 , P. 74-83
Fonte: | 2006 Elsevier Inc. All rights reserved