Data/hora: 10/06/2024 15:07:58

Provedor de dados: 	Institutional Archive of Ifreme (ArchiMer)	
País: 	France	
Título: 	Cellular responses of Pacific oyster ( Crassostrea gigas ) gametes exposed in vitro to polystyrene nanoparticles	
Autores: 	Gonzalez-fernandez, Carmen; Tallec, Kevin; Le Goic, Nelly; Lambert, Christophe; Soudant, Philippe; Huvet, Arnaud; Suquet, Marc; Berchel, Mathieu; Paul-pont, Ika.	
Data: 	2018-10	
Ano: 	2018	
Palavras-chave: 	Nanoplastics; Oysters; Gametes; Cellular responses.	
Resumo: 	While the detection and quantification of nano-sized plastic in the environment remains a challenge, the growing number of polymer applications mean that we can expect an increase in the release of nanoplastics into the environment by indirect outputs. Today, very little is known about the impact of nano-sized plastics on marine organisms. Thus, the objective of this study was to investigate the toxicity of polystyrene nanoplastics (NPs) on oyster (Crassostrea gigas) gametes. Spermatozoa and oocytes were exposed to four NPs concentrations ranging from 0.1 to 100 mg L−1 for 1, 3 and 5 h. NPs coated with carboxylic (PS-COOH) and amine groups (PS-NH2) were used to determine how surface properties influence the effects of nanoplastics. Results demonstrated the adhesion of NPs to oyster spermatozoa and oocytes as suggested by the increase of relative cell size and complexity measured by flow-cytometry and confirmed by microscopy observations. A significant increase of ROS production was observed in sperm cells upon exposure to 100 mg L−1 PS-COOH, but was not observed with PS-NH2, suggesting a differential effect according to the NP-associated functional group. Altogether, these results demonstrate that the effects of NPs occur rapidly, are complex and are possibly associated with the cellular eco-corona, which could modify NPs behaviour and toxicity.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	https://archimer.ifremer.fr/doc/00443/55459/56976.pdf

DOI:10.1016/j.chemosphere.2018.06.039

https://archimer.ifremer.fr/doc/00443/55459/	
Editor: 	Pergamon-elsevier Science Ltd	
Formato: 	application/pdf	
Fonte: 	Chemosphere (0045-6535) (Pergamon-elsevier Science Ltd), 2018-10 , Vol. 208 , P. 764-772	
Fonte: 	2018 Published by Elsevier Ltd.

info:eu-repo/semantics/openAccess

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