Data/hora: 29/05/2024 02:50:47

Provedor de dados: 	Institutional Archive of Ifreme (ArchiMer)	
País: 	France	
Título: 	Detection of ostreid herpesvirus-1 (OsHV-1) by PCR using a rapid and simple method of DNA extraction from oyster larvae	
Autores: 	Batista, Frederico; Taris, Nicolas; Boudry, Pierre; Renault, Tristan.	
Data: 	2005-04	
Ano: 	2005	
Palavras-chave: 	DNA extraction; Detection; Larvae; Oyster; OsHV 1; Herpesvirus.	
Resumo: 	A DNA extraction procedure was developed for the detection of ostreid herpesvirus-1 (OsHV-1) using the polymerase chain reaction (PCR) in oyster larvae. The DNA extraction procedure developed was tested on 8 larval samples. Abnormal nuclei with characteristic features associated with OsHV-1 infections were only observed in samples in which the viral DNA was detected by PCR. A previously described competitive PCR method was applied to detect inhibition during PCR reactions. The results show that the method can be used on small amounts of oyster larvae (3 mg) for the detection of OsHV-1 DNA by PCR.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/2005/publication-2916.pdf	
Editor: 	Inter-Research	
Relação: 	http://archimer.ifremer.fr/doc/00000/2916/	
Formato: 	application/pdf	
Fonte: 	Diseases of aquatic organisms (0177-5103) (Inter-Research), 2005-04 , Vol. 64 , N. 1 , P. 1-4	
Fonte: 	Inter-Research