Data/hora: 17/06/2024 07:04:53

Provedor de dados: 	5	
País: 	France	
Título: 	Essai d'aptitude par comparaison Inter-analystes pour l'évaluation des performances en identification et dénombrement du phytoplancton marin. Rapport d'évaluation des compétences des analystes du phytoplancton dans le cadre du réseau d'observation et de surveillance du phytoplancton et de l'hydrologie (REPHY) mis en oeuvre par l'Ifremer	
Autores: 	Neaud-masson, Nadine; Brun, Melanie; Chomerat, Nicolas.	
Data: 	2018-04	
Ano: 	2018	
Palavras-chave: 	Phytoplancton; Comparaison Inter-Laboratoire; CIL; Comparaison Inter-Analyste; CIA; Essai d’aptitude; Phytoplankton; Inter-Laboratory Comparison; CIL; Inter-Analyst Comparison; CIA; Aptitude Test; International Phytoplankton Inter-comparison; IPI.	
Resumo: 	The determination of phytoplankton species and the estimation of their abundance in the context of the Phytoplankton and Hydrology Monitoring and Surveillance Network, implemented by Ifremer (REPHY), is based in 2017 on 21 analysts spread over 12 sites. Proficiency testing by inter-laboratory or inter-analyst comparison is used to evaluate the performance of laboratories or analysts on specific skills. An International Phytoplankton Inter-comparison (IPI) is organized annually by the Marine Institute Phytoplankton unit of Galway (Ireland), to evaluate marine phytoplankton identification and enumeration performance. The IPI is implementing its inter-comparison exercises through the Marine Institute Ireland in cooperation with the Intergovernmental Oceanographic Commission of UNESCO (IOC) through its Science and Communication Centre on Harmful Algae at the University of Copenhagen, Denmark. The UK NE Atlantic Marine Biological Analytical Quality Control Scheme (NMBAQC) is one of their partners. In 2017, nine REPHY analysts were enrolled individually to participate in the IPI. The samples sent to the relevant analysts were used to also assess the skills of non-IPI analysts. A total of 16 on 21 REPHY analysts reported their results. This report describes the progress of the IPI and the Ifremer complementary test (Inter-Analyst Comparison) and presents only the results obtained for the Ifremer test. The objective is to provide elements to all REPHY staff in order to maintain a continuous improvement of practices and to determine actions required for this improvement. Regarding the identification of taxa, with 98.6% of correct results and all genera correctly identified by fifteen participants, the test confirms the skills of the analysts. The difficulties of specific identifications mainly concern armoured (thecate) dinoflagellates. Epifluorescence microscopy is a technique that allows the observation of dinoflagellate theca without destruction of the material, and the transition from phase contrast to epifluorescence can be done during the analysis, which is an advantage over the manual dissection (destructive) of the cells. To date, five laboratories out of the twelve implantations of the REPHY monitoring network have a microscope equipped with an epifluorescence module. It is planned to gradually equip all the laboratories of our network. The statistical analysis of the quantitative results is based on the calculation of the z score as a performance measure, as defined in the ISO 13528: 2015 standard. Regarding these results, 95.1% of z scores are within the tolerance limits, which is overall very satisfactory. Statistical analysis highlights the importance of the number of participants. Indeed, sixteen REPHY analysts, who had 21 analysts in June 2017, participated while a minimum of 18 participants was needed to make the uncertainty of the assigned value negligible. The exercise runs from June to August. This period is not very conducive to the mobilization of staff, who sees his load plan increase due to absences for leave. However, it is not possible to change that since we are dependent on the organization of the Marine Institute Galway.	
Tipo: 	Text	
Idioma: 	Francês	
Identificador: 	https://archimer.ifremer.fr/doc/00436/54797/56255.pdf

https://archimer.ifremer.fr/doc/00436/54797/	
Formato: 	application/pdf	
Fonte: 	2018 Aquaref, Ifremer

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