Data/hora: 09/06/2024 11:28:45

Provedor de dados: | Institutional Archive of Ifreme (ArchiMer)
País: | France
Título: | Towards the development of clonal lines in the European sea bass (Dicentrarchus labrax L.): application of uniparental reproduction techniques with an insight into sea bass eggs
Autores: | Colleter, Julie.
Data: | 2015-02-13
Ano: | 2015
Palavras-chave: | Dicentrarchus labrax; Lignées clonales; Androgenèse; Gynogenèse; MAAs; Gadusol; Marqueurs microsatellites; Homozygotie; Hétérozygotie; Taux de recombinaison.; Dicentrarchus labrax; Clonal lines; Androgenesis; Gynogenesis; MAAs; Gadusol; Microsatellite markers; Homozygosity; Heterozygosity; Recombination rate.
Resumo: | Clonal lines are a powerful scientific tool for improved genetic characterization of organisms used in research. Inbred fish lines can be produced in only two generations using uniparental reproduction techniques. Androgenesis, achieved with variable success in several freshwater species, has been attempted in the European sea bass (Dicentrarchus labrax L), a marine fish of commercial and scientific interest. The low yields of progenies inheriting only the paternal genome after UV-irradiation of eggs led to considerations on the occurrence of UV screening compounds in pelagic eggs. Mycosporine-like amino acids and gadusol were found in many marine and freshwater organisms, but their occurrence in fish eggs was not clearly related to a behavioral pattern and while gadusol appeared in higher proportions in pelagic marine eggs compared to benthic species, this statement did not apply in freshwater, and moreover the kind of compounds was related to phylogeny. Further studies on DNA photorepair could enlighten hypotheses to understand the mechanisms underlying the disparate results obtained in inducing androgenesis in different fish species. Gynogenesis was reported successful to produce clonal founders in the sea bass, but high numbers of meiotic individuals contaminating fully homozygous progenies highlighted the need for efficient DNA markers to distinguish mitotic gynogenetic individuals. Furthermore, gonad development was highly delayed in gynogenetic progenies enhancing the difficulties to produce clonal lines. A high variability between individuals in the success of uniparental reproduction brought out gamete characterization and quality as a prerequisite.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00300/41138/40326.pdf

http://archimer.ifremer.fr/doc/00300/41138/

Editor: | Université Montpellier 2
Relação: | info:eu-repo/grantAgreement/EC/FP7/262336/EU//AQUA EXCEL
Formato: | application/pdf

Fonte:

2015 Université de Montpellier

info:eu-repo/semantics/openAccess

restricted use