Data/hora: 09/06/2024 07:34:24

Provedor de dados: | Institutional Archive of Ifreme (ArchiMer)
País: | France
Título: | Exploration des communautés virales thermophiles dans les écosystèmes chauds des Terres australes et antarctiques françaises
Autores: | Parikka, Kaarle Joonas.
Data: | 2013-03-28
Ano: | 2013
Palavras-chave: | Bactérie; Archée; Induction virale; Lysogénie; Source chaude; Abondance; Bacteria; Archaea; Phage; Thermophile; Viral induction; Lysogeny; Hot spring; Abundance.
Resumo: | Viruses thrive in all types of ecosystems where life is found. They represent the most abundant biological entity of our biosphere. Though several studies have been conducted on viral abundance and dynamics in mesophilic aquatic ecosystems, these aspects remain largely unexplored in extremophilic environments, such as hot springs. In this study, prokaryotic and associated viral communities of the French Southern and Antarctic Lands hot springs were explored. First, prokaryotic cells and Virus-like particles (VLP) were enumerated in several terrestrial and inshore hot springs. The results reveal an abundance of 105 - 106 particles/ml in both types of hot springs studied. The virus-to-prokaryote ratios (VPR) were generally low, confirming thus actual knowledge in these types of ecosystems. The morphological diversity of VLP was then studied in raw samples as well as in enrichment cultures containing Thermococcales and Thermotogales. Several isolates obtained from these samples were then screened for viral particles which led to the discovery and description of a temperate phage (GTV1) of a thermophilic bacterium belonging to the genus Geobacillus. The effect of NaCl and anoxic stress on the viral production was studied. The genomic characterization of the GTV1 was started and revealed a 38441 bp genome with 71predicted ORF. Finally, microbial diversity studies led also to the discovery of a new extremely thermophilic bacterium, Calditerricola clavaformis sp.nov.
Tipo: | Text
Idioma: | Francês

Identificador:

https://archimer.ifremer.fr/doc/00609/72139/70891.pdf

https://archimer.ifremer.fr/doc/00609/72139/

Editor: | Université de Bretagne Occidentale
Formato: | application/pdf

Fonte:

info:eu-repo/semantics/openAccess

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