Data/hora: 09/06/2024 06:42:57

Provedor de dados: | Institutional Archive of Ifreme (ArchiMer)
País: | France
Título: | Disturbance induced decoupling between host genetics and composition of the associated microbiome
Autores: | Wegner, Karl Mathias; Volkenborn, Nils; Peter, Hannes; Eiler, Alexander.
Data: | 2013-11
Ano: | 2013
Palavras-chave: | Microbiota; Population structure; Stress; Pathogen; Biological invasion; Pacific oyster; Crassostrea gigas; Vibrio; Mycoplasma.
Resumo: | Background  Studies of oyster microbiomes have revealed that a limited number of microbes, including pathogens, can dominate microbial communities in host tissues such as gills and gut. Much of the bacterial diversity however remains underexplored and unexplained, although environmental conditions and host genetics have been implicated. We used 454 next generation 16S rRNA amplicon sequencing of individually tagged PCR reactions to explore the diversity of bacterial communities in gill tissue of the invasive Pacific oyster Crassostrea gigas stemming from genetically differentiated beds under ambient outdoor conditions and after a multifaceted disturbance treatment imposing stress on the host. Results  While the gill associated microbial communities in oysters were dominated by few abundant taxa (i.e. Sphingomonas, Mycoplasma) the distribution of rare bacterial groups correlated to relatedness between the hosts under ambient conditions. Exposing the host to disturbance broke apart this relationship by removing rare phylotypes thereby reducing overall microbial diversity. Shifts in the microbiome composition in response to stress did not result in a net increase in genera known to contain potentially pathogenic strains. Conclusion  The decrease in microbial diversity and the disassociation between population genetic structure of the hosts and their associated microbiome suggest that disturbance (i.e. stress) may play a significant role for the assembly of the natural microbiome. Such community shifts may in turn also feed back on the course of disease and the occurrence of mass mortality events in oyster populations.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00171/28202/26430.pdf

DOI:10.1186/1471-2180-13-252

Editor: | Biomed Central Ltd
Relação: | http://archimer.ifremer.fr/doc/00171/28202/
Formato: | application/pdf
Fonte: | Bmc Microbiology (1471-2180) (Biomed Central Ltd), 2013-11 , Vol. 13 , P. -
Fonte: | 2013 Wegner et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.