Data/hora: 08/06/2024 17:30:27

Provedor de dados: | 5
País: | France
Título: | Exploring the Impact of the Biofloc Rearing System and an Oral WSSV Challenge on the Intestinal Bacteriome of Litopenaeus vannamei
Autores: | Pilotto, Mariana R.; Goncalves, Andre N. A.; Vieira, Felipe N.; Seifert, Walter Q.; Bachere, Evelyne; Rosa, Rafael D.; Perazzolo, Luciane M.
Data: | 2018-09
Ano: | 2018
Palavras-chave: | Biofloc technology (BFT); Penaeid shrimp; Gut microbiota; White spot syndrome virus; 16S rRNA gene sequencing.
Resumo: | We provide a global overview of the intestinal bacteriome of Litopenaeus vannamei in two rearing systems and after an oral challenge by the White spot syndrome virus (WSSV). By using a high-throughput 16S rRNA gene sequencing technology, we identified and compared the composition and abundance of bacterial communities from the midgut of shrimp reared in the super-intensive biofloc technology (BFT) and clear seawater system (CWS). The predominant bacterial group belonged to the phylum Proteobacteria, followed by the phyla Bacteroidetes, Actinobacteria, and Firmicutes. Within Proteobacteria, the family Vibrionaceae, which includes opportunistic shrimp pathogens, was more abundant in CWS than in BFT-reared shrimp. Whereas the families Rhodobacteraceae and Enterobacteriaceae accounted for almost 20% of the bacterial communities of shrimp cultured in BFT, they corresponded to less than 3% in CWS-reared animals. Interestingly, the WSSV challenge dramatically changed the bacterial communities in terms of composition and abundance in comparison to its related unchallenged group. Proteobacteria remained the dominant phylum. Vibrionaceae was the most affected in BFT-reared shrimp (from 11.35 to 20.80%). By contrast, in CWS-reared animals the abundance of this family decreased from 68.23 to 23.38%. Our results provide new evidence on the influence of both abiotic and biotic factors on the gut bacteriome of aquatic species of commercial interest.
Tipo: | Text
Idioma: | Inglês

Identificador:

https://archimer.ifremer.fr/doc/00454/56546/58256.pdf

DOI:10.3390/microorganisms6030083

https://archimer.ifremer.fr/doc/00454/56546/

Editor: | Mdpi
Formato: | application/pdf
Fonte: | Microorganisms (2076-2607) (Mdpi), 2018-09 , Vol. 6 , N. 3 , P. 83 (16p.)

Fonte:

2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

info:eu-repo/semantics/openAccess

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