Data/hora: 09/06/2024 06:08:30

Provedor de dados: 	Institutional Archive of Ifreme (ArchiMer)	
País: 	France	
Título: 	The oyster vasa-like gene: a specific marker of the germline in Crassostrea gigas	
Autores: 	Fabioux, Caroline; Pouvreau, Stephane; Leroux, Frederique; Huvet, Arnaud.	
Data: 	2004-03	
Ano: 	2004	
Palavras-chave: 	RNA helicase; DEAD box; Vasa; Germline; Gametogenesis; Oyster.	
Resumo: 	The vasa gene is,a key determinant for germline formation in eukaryotes. This gene, highly conserved through evolution, encodes a RNA helicase protein member of the DEAD-box family. To understand the germline formation in oyster, we report here the isolation and the characterization of a vasa orthologue in Crassostrea gigas (Oyvlg). OyVLG contained the eight consensus domains of the DEAD-box including those providing RNA unwinding activity. The expression pattern of Oyvlg was examined in adult oyster tissues at different reproductive stages. Its expression was restricted to germline cells both in males and females, including germinal stem cells and auxiliary cells. The expression of Oyvlg, strongest in early germ cells, decreased as the maturation proceeded. These data and the evolutionary conservation observed suggested the role of Oyvlg in germline development. Oyvlg is the first germ cell specific marker in oyster and will be very useful in studies of oyster germline formation.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/2004/publication-646.pdf

DOI:10.1016/j.bbrc.2004.01.145	
Editor: 	Elsevier	
Relação: 	http://archimer.ifremer.fr/doc/00000/646/	
Formato: 	application/pdf	
Fonte: 	Biochemical and Biophysical Research Communications (0006-291X) (Elsevier), 2004-03 , Vol. 315 , N. 4 , P. 897-904	
Fonte: 	2004 Elsevier Inc. All rights reserved