Data/hora: 09/06/2024 01:50:30

Provedor de dados: | 5
País: | France
Título: | Outer membrane vesicles are vehicles for the delivery of Vibrio tasmaniensis virulence factors to oyster immune cells
Autores: | Vanhove, Audrey; Duperthuy, Marylise; Charriere, Guillaume; Le Roux, Frederique; Goudenege, David; Gourbal, Benjamin; Kieffer-jaquinod, Sylvie; Coute, Yohann; Wai, Sun Nyunt; Destoumieux-garzon, Delphine.
Data: | 2015-04
Ano: | 2015
Resumo: | Vibrio tasmaniensis LGP32, a facultative intracellular pathogen of oyster haemocytes, was shown here to release outer membrane vesicles (OMVs) both in the extracellular milieu and inside haemocytes. Intracellular release of OMVs occurred inside phagosomes of intact haemocytes having phagocytosed few vibrios as well as in damaged haemocytes containing large vacuoles heavily loaded with LGP32. The OMV proteome of LGP32 was shown to be rich in hydrolases (25%) including potential virulence factors such as proteases, lipases, phospholipases, haemolysins and nucleases. One major caseinase/gelatinase named Vsp for vesicular serine protease was found to be specifically secreted through OMVs in which it is enclosed. Vsp was shown to participate in the virulence phenotype of LGP32 in oyster experimental infections. Finally, OMVs were highly protective against antimicrobial peptides, increasing the minimal inhibitory concentration of polymyxin B by 16-fold. Protection was conferred by OMV titration of polymyxin B but did not depend on the activity of Vsp or another OMV-associated protease. Altogether, our results show that OMVs contribute to the pathogenesis of LGP32, being able to deliver virulence factors to host immune cells and conferring protection against antimicrobial peptides.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00199/31009/29459.pdf

DOI:10.1111/1462-2920.12535

http://archimer.ifremer.fr/doc/00199/31009/

Editor: | Wiley-blackwell
Formato: | application/pdf
Fonte: | Environmental Microbiology (1462-2912) (Wiley-blackwell), 2015-04 , Vol. 17 , N. 4 , P. 1152-1165

Fonte:

2014 Society for Applied Microbiology and John Wiley &amp; Sons Ltd

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