Data/hora: 11/06/2024 10:24:37

Provedor de dados: | 5
País: | France
Título: | Determination of reduced sulfur compounds by high-performance liquid chromatography in hydrothermal seawater and body fluids from Riftia pachyptila
Autores: | Gru, Cecile; Sarradin, Pierre-marie; Legoff, Hélène; Narcon, Sandrine; Caprais, Jean-claude; Lallier, Francois.
Data: | 1998-06
Ano: | 1998
Palavras-chave: | Sulfide; Sulfite; Thiosulfate; Cysteine; Glutathione; Hydrothermal seawater; Riftia pachyptila; Biological fluids.
Resumo: | This paper describes a method for the determination of reduced sulfur compounds in hydrothermal seawater and body fluids from the hydrothermal tube worm Riftia pachyptila. Sulfur is a hey component of the hydrothermal ecosystem based on chemosynthesis, Sulfur compounds were derivatized at pH 8 (4.3 for sulfide in biological fluid) with a fluorescent reagent, monobromobimane, and separated by reverse-phase HPLC. The eluted compounds were detected by spectrofluorimetry. This method allowed: the quantitative analysis of sulfide, sulfite, thiosulfate, cysteine and glutathione in seawater, vascular blood and coelomic fluids from R, pachyptila, The detection limits were in the 0.1 mu mol l(-1) range with a precision lower than 10%, The method has been applied to hydrothermal seawater. The organisms are distributed along a gradient of sulfide (produced by the vent) and thiosulfate. Analysis of biological fluid was performed with a new sample treatment allowing the analysis of total sulfide (free and bound to haemoglobin) with results comparable to published methods.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00186/29763/28203.pdf

DOI:10.1039/a800032h

Editor: | Royal Soc Chemistry
Relação: | http://archimer.ifremer.fr/doc/00186/29763/
Formato: | application/pdf
Fonte: | Analyst (0003-2654) (Royal Soc Chemistry), 1998-06 , Vol. 123 , N. 6 , P. 1289-1293