Data/hora: 13/06/2024 02:15:30

Provedor de dados: | 5
País: | France
Título: | Needle in a haystack: involvement of the copepod Paracartia grani, in the life-cycle of the oyster pathogen Marteilia refringens
Autores: | Audemard, Corinne; Le Roux, Frédérique; Barnaud, Antoine; Collins, Catherine; Sautour, B; Sauriau, Pierre-guy; De Montaudouin, X; Coustau, C; Combes, C; Berthe, Franck.
Data: | 2002-09
Ano: | 2002
Palavras-chave: | Experimental transmission; In situ hybridization; PCR; Paracartia grani; Ostrea edulis; Marteilia refringens.
Resumo: | Marteilia refringens is a major pathogen of the European flat oyster, Ostrea edulis Linnaeus. Since its description, the lifecycle of this protozoan parasite has eluded discovery. Attempts to infect oysters experimentally have been unsuccessful and led to the hypothesis of a complex life-cycle involving several hosts. Knowledge of this life-cycle is of central importance in order to manage oyster disease. However, the exploration of M. refringens life-cycle has been previously limited by the detection tools available and the tremendous number of species to be screened in enzootic areas. In this study, these two restrictions were circumvented by the use of both molecular detection tools and a mesocosm with low biodiversity. Screening of the entire fauna of the pond for AT. refringens DNA was systematically undertaken using PCR. Here, we show that the copepod Paracartia (Acartia) grani is a host of M. refringens. Not only was DNA of M. refringens consistently detectcd in P. grani but also the presence of the parasite in the ovarian tissues was demonstrated using in situ hybridization. Finally, successful experimental transmissions provided evidence that P. grani can be infected from infected flat oysters.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2002/publication-2778.pdf

DOI:10.1017/S0031182001001111

Editor: | Cambridge University Press
Relação: | http://archimer.ifremer.fr/doc/00000/2778/
Formato: | application/pdf
Fonte: | Parasitology (0031-1820) (Cambridge University Press), 2002-09 , Vol. 124 , P. 315-323
Fonte: | Cambridge University Press