Data/hora: 01/06/2024 02:45:32

Provedor de dados: 	Institutional Archive of Ifreme (ArchiMer)	
País: 	France	
Título: 	Ovatoxines: Purification à partir d’Ostreopsis cf. ovata en culture et niveaux d’accumulation dans les produits de la mer	
Autores: 	Brissard, Charline.	
Data: 	2014-10-21	
Ano: 	2014	
Palavras-chave: 	Ostreopsis ovata; Ovatoxines; Purification; Contamination des produits de la mer; Chromatographie liquide/spectrométrie de masse.; Ostreopsis ovata; Ovatoxins; Purification; Seafood contamination; Liquid chromatography / mass spectrometry.	
Resumo: 	Dinoflagellates of genus Ostreopsis are known in tropical areas to provoke food intoxication, after palytoxin accumulation in seafood. Probably in link with climatic changes, Ostreopsis sp. is increasingly present in French Mediterranean since 2006. In the context of the PhD, the studies in natural environment, in summer 2011 and 2012, showed that: i) Ostreopsis cf. ovata strain presented a complex toxinic profile, including palytoxin and especially ovatoxins (OVTX-a, -b, c, -d, -e). A new analog (OVTX-h) was detected; ii) cell and toxinic concentrations decreased with depth; iii) different seafood accumulated toxins, which could represent real risk for bathers and consumers. Because there are no standards for OVTXs, one of the PhD goals was to develop a OVTXs purification protocol from Ostreopsis cf. ovata extracts. For this purpose, optimal culture conditions were investigated, allowing a maximal production of OVTXs (300 pg/cell). Different extraction and concentration steps developed had permitted to obtain a toxin rich fraction. Finale purification was performed using liquid chromatography/mass spectrometry/ UV detection, using Uptisphere C18-TF column. PhD researches have allowed to acquire data, which contribute to analysis of sanitary impact, since nowadays, at international level, there are just few data allowing to establish Ostreopsis sp. alert and sanitary security threshold.	
Tipo: 	Text	
Idioma: 	Francês	
Identificador: 	http://archimer.ifremer.fr/doc/00255/36613/35179.pdf

http://archimer.ifremer.fr/doc/00255/36613/	
Editor: 	Université de Nantes. Faculté des sciences et des techniques. Ecole doctorale Végétal-Environnement-Nutrition-Agroalimentaire-Mer VENAM	
Formato: 	application/pdf	
Fonte: 	info:eu-repo/semantics/openAccess

restricted use