Data/hora: 05/06/2024 10:19:48

Provedor de dados: | Institutional Archive of Ifreme (ArchiMer)
País: | France
Título: | Expression of candidate genes related to metabolism, immunity and cellular stress during massive mortality in the American oyster Crassostrea virginica larvae in relation to biochemical and physiological parameters
Autores: | Genard, Bertrand; Moraga, Dario; Pernet, Fabrice; David, Elise; Boudry, Pierre; Tremblay, Rejean.
Data: | 2012-05
Ano: | 2012
Palavras-chave: | Massive mortality; Relative gene expression; Metabolism; Immunity; Cellular stress; Larvae.
Resumo: | Quantification of mRNA of genes related to metabolism, immunity and cellular stress was examined in relation to a massive mortality event during the culture of American oyster larvae, Crassostrea virginica which was probably, in regard to previous microbiological analysis, induced by vibrio infection. To document molecular changes associated with the mortality event, mRNA levels were compared to biochemical and physiological data, previously described in a companion paper. Among the 18 genes studied, comparatively to the antibiotic control, 10 showed a lower relative gene expression when the massive mortality occurred. Six of them are presumed to be related to metabolism, corroborating the metabolic depression associated with the mortality event suggested by biochemical and physiological analyses. Relationships between the regulation of antioxidant enzyme activities, lipid peroxidation, and the mRNA abundance of genes linked to oxidative stress, cytoprotection, and immune response are also discussed. Finally, we observed an increase in the transcript abundance of two genes involved in apoptosis and cell regulation simultaneously with mortality, suggesting that these processes might be linked.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00075/18585/16138.pdf

DOI:10.1016/j.gene.2012.02.021

Editor: | Elsevier Science Bv
Relação: | http://archimer.ifremer.fr/doc/00075/18585/
Formato: | application/pdf
Fonte: | Gene (0378-1119) (Elsevier Science Bv), 2012-05 , Vol. 499 , N. 1 , P. 70-75
Fonte: | 2012 Published by Elsevier B.V. All rights reserved