Data/hora: 03/06/2024 16:33:24

Provedor de dados: | Institutional Archive of Ifreme (ArchiMer)
País: | France
Título: | Preparation and characterization of monoclonal antibodies against neoplastic hemocytes of Mytilus edulis (Bivalvia)
Autores: | Noel, Danielle; Boulo, Viviane; Chagot, Dominique; Mialhe, Eric; Paolucci, Francis; Clavies, Christine; Hervaud, Eric; Elston, Ralph.
Data: | 1991-03
Ano: | 1991
Resumo: | Mytilus edulis develop progressive and fatal hemocytic neoplasia. We have generated monoclonal antibodies using neoplastic hemocytes from highly infected individuals collected from Puget Sound in Washington State (USA). Twenty-seven monoclonal antibodies against neoplatic cell antigens were obtained. Specificity of 2 purified antibodies, 14F1 and 16G10, was demonstrated by immunofluorescence when  incubated with neoplastic hemocytes, whereas these anbidodies did not react with normal cells. This specificity was confirmed by immunogold labelling and electron microscopic visualization. The technique permitted localization of the epitopes recognized by the monoclonal antibodies on the plasma membrane. These monoclonal antibodies are suitable reagents for establishment of immunoassays for diagnosis and quantification of hemic neoplasia in the mussel.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/1991/publication-2757.pdf

DOI:10.3354/dao010051

Editor: | Inter-research
Relação: | http://archimer.ifremer.fr/doc/00000/2757/
Formato: | application/pdf
Fonte: | Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 1991-03 , Vol. 10 , N. 1 , P. 51-58
Fonte: | Inter-Research/Printed in Germany