Data/hora: 08/06/2024 18:32:50

Provedor de dados: | 5
País: | France
Título: | Field enhanced bacterial sample stacking in isotachophoresis using wide-bore capillaries
Autores: | Oukacine, Farid; Quirino, Joselito P.; Destoumieux-garzon, Delphine; Cottet, Herve.
Data: | 2012-12
Ano: | 2012
Palavras-chave: | Capillary zone electrophoresis; Isotachophoresis; Bacteria; Electrokinetic injection; Field-enhanced sample injection.
Resumo: | The isotachophoretic analysis of different bacterial strains was studied using capillaries with different internal diameters from 50 to 250 mu m. Several injection modes were investigated and compared in order to improve the limit of detection of bacteria by capillary isotachophoresis. A system suitability test obtained from the separation voltage was developed to ensure reliable results. As expected, the use of wider bore capillaries improved the analytical sensitivity of the isotachophoretic method when compared to the 50 mu m capillary. With the optimized conditions, the isotachophoretic method presented in this work allows the quantification of Erwinia carotovora (Gram negative bacteria) with a limit of detection as low as similar to 3000 cells mL(-1). The proposed methodology does not require any additive in the electrolyte such a fluorescent or chromophoric dye to reach these limits of detection. (C) 2012 Elsevier BM. All rights reserved.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00115/22636/20596.pdf

DOI:10.1016/j.chroma.2012.10.026

Editor: | Elsevier Science Bv
Relação: | http://archimer.ifremer.fr/doc/00115/22636/
Formato: | application/pdf
Fonte: | Journal Of Chromatography A (0021-9673) (Elsevier Science Bv), 2012-12 , Vol. 1268 , P. 180-184
Fonte: | 2012 Elsevier B.V. All rights reserved.