Data/hora: 01/06/2024 02:22:06

Provedor de dados: | Institutional Archive of Ifreme (ArchiMer)
País: | France
Título: | Marteilia refringens and Marteilia pararefringens sp. nov. are distinct parasites of bivalves and have different European distributions
Autores: | Kerr, R.; Ward, G. M.; Stentiford, G. D.; Alfjorden, A.; Mortensen, S.; Bignell, J. P.; Feist, S. W.; Villalba, A.; Carballal, M. J.; Cao, A.; Arzul, Isabelle; Ryder, D.; Bass, D.
Data: | 2018-09
Ano: | 2018
Palavras-chave: | Marteilia refringens; Marteilia pararefringens; ITS1 rDNA; IGS rDNA; Paramyxida; Ascetosporea; Mytilus edulis; Ostrea edulis.
Resumo: | Marteilia refringens causes marteiliosis in oysters, mussels and other bivalve molluscs. This parasite previously comprised two species, M. refringens and Marteilia maurini, which were synonymized in 2007 and subsequently referred to as M. refringens ‘O-type’ and ‘M-type’. O-type has caused mass mortalities of the flat oyster Ostrea edulis. We used high throughput sequencing and histology to intensively screen flat oysters and mussels (Mytilus edulis) from the UK, Sweden and Norway for infection by both types and to generate multi-gene datasets to clarify their genetic distinctiveness. Mussels from the UK, Norway and Sweden were more frequently polymerase chain reaction (PCR)-positive for M-type (75/849) than oysters (11/542). We did not detect O-type in any northern European samples, and no histology-confirmed Marteilia-infected oysters were found in the UK, Norway and Sweden, even where co-habiting mussels were infected by the M-type. The two genetic lineages within ‘M. refringens’ are robustly distinguishable at species level. We therefore formally define them as separate species: M. refringens (previously O-type) and Marteilia pararefringens sp. nov. (M-type). We designed and tested new Marteilia-specific PCR primers amplifying from the 3’ end of the 18S rRNA gene through to the 5.8S gene, which specifically amplified the target region from both tissue and environmental samples.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00444/55566/57177.pdf

http://archimer.ifremer.fr/doc/00444/55566/57178.pdf

DOI:10.1017/S003118201800063X

http://archimer.ifremer.fr/doc/00444/55566/

Editor: | Cambridge Univ Press
Relação: | info:eu-repo/grantAgreement/EC/FP7/678589/EU//VIVALDI
Formato: | application/pdf
Fonte: | Parasitology (0031-1820) (Cambridge Univ Press), 2018-09 , Vol. 145 , N. 11 , P. 1483-1492

Fonte:

Crown Copyright and Cambridge University Press. Published by Cambridge University Press 2018. This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http:// creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

info:eu-repo/semantics/openAccess

restricted use