Data/hora: 12/06/2024 14:40:48

Provedor de dados: | 5
País: | France
Título: | In situ QCM DNA-biosensor probe modification
Autores: | Lazerges M, M; Perrot, H; Zeghib N, N; Antoine, Elisabeth; Compere, Chantal.
Data: | 2006-12
Ano: | 2006
Palavras-chave: | QCM; DNA; Biosensor.
Resumo: | We report on a probe modification of a quartz crystal microbalance (QCM) DNA-biosensor that permits to reversibly change the DNA sequence detected. A QCM DNA-biosensor was designed by immobilization of a 20-base DNA-disulfide probe on the gold-covered quartz surface of a 27 MHz microbalance (9 MHz, third overtone). After immobilization on the gold covered quartz surface, this probe was modified by hybridization with a 45-base DNA that includes the complementary 20-base sequence, a 5-base spacer and a non-complementary 20-base sequence. The non-complementary sequence constitutes a new probe, different from the DNA-disulfide probe, that permits the detection of a new DNA target. As this 45 bases DNA is changeable by dehybridization, successive different DNA targets can be detected. Kinetics and thermodynamic studies of the DNA-disulfide and modified biosensors indicate that the modified biosensor is as sensitive, selective, fast, renewable and reproducible as the DNA-disulfide biosensor, but with a higher hybridization ratio. This modification method offers wider investigation field and practical and economic advantages to DNA-biosensors based on irreversible immobilization of DNA probes on solid substrate. (c) 2006 Elsevier B.V. All rights reserved.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2006/publication-1985.pdf

DOI:10.1016/j.snb.2006.02.024

Editor: | Elsevier
Relação: | http://archimer.ifremer.fr/doc/00000/1985/
Formato: | application/pdf
Fonte: | Sensors and Actuators B: Chemical (0925-4005) (Elsevier), 2006-12 , Vol. 120 , N. 1 , P. 329-337
Fonte: | 2006 Elsevier B.V.