Data/hora: 10/06/2024 17:12:15

Provedor de dados: 	Institutional Archive of Ifreme (ArchiMer)	
País: 	France	
Título: 	In situ QCM DNA-biosensor probe modification	
Autores: 	Lazerges M, M; Perrot, H; Zeghib N, N; Antoine, Elisabeth; Compere, Chantal.	
Data: 	2006-12	
Ano: 	2006	
Palavras-chave: 	QCM; DNA; Biosensor.	
Resumo: 	We report on a probe modification of a quartz crystal microbalance (QCM) DNA-biosensor that permits to reversibly change the DNA sequence detected. A QCM DNA-biosensor was designed by immobilization of a 20-base DNA-disulfide probe on the gold-covered quartz surface of a 27 MHz microbalance (9 MHz, third overtone). After immobilization on the gold covered quartz surface, this probe was modified by hybridization with a 45-base DNA that includes the complementary 20-base sequence, a 5-base spacer and a non-complementary 20-base sequence. The non-complementary sequence constitutes a new probe, different from the DNA-disulfide probe, that permits the detection of a new DNA target. As this 45 bases DNA is changeable by dehybridization, successive different DNA targets can be detected. Kinetics and thermodynamic studies of the DNA-disulfide and modified biosensors indicate that the modified biosensor is as sensitive, selective, fast, renewable and reproducible as the DNA-disulfide biosensor, but with a higher hybridization ratio. This modification method offers wider investigation field and practical and economic advantages to DNA-biosensors based on irreversible immobilization of DNA probes on solid substrate. (c) 2006 Elsevier B.V. All rights reserved.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/2006/publication-1985.pdf

DOI:10.1016/j.snb.2006.02.024	
Editor: 	Elsevier	
Relação: 	http://archimer.ifremer.fr/doc/00000/1985/	
Formato: 	application/pdf	
Fonte: 	Sensors and Actuators B: Chemical (0925-4005) (Elsevier), 2006-12 , Vol. 120 , N. 1 , P. 329-337	
Fonte: 	2006 Elsevier B.V.