Data/hora: 02/06/2024 05:00:59

Provedor de dados: 	5	
País: 	France	
Título: 	Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment	
Autores: 	Morvezen, Romain; Cornette, Florence; Charrier, Gregory; Guinand, Bruno; Lapegue, Sylvie; Boudry, Pierre; Laroche, Jean.	
Data: 	2013-07	
Ano: 	2013	
Palavras-chave: 	Microsatellites; Polymerase chain reaction; Parentage; Assignment; Bivalve mollusc.	
Resumo: 	We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00137/24822/22907.pdf

DOI:10.1051/alr/2013052	
Editor: 	Edp Sciences S A	
Relação: 	http://archimer.ifremer.fr/doc/00137/24822/

info:eu-repo/grantAgreement/EC/FP7/245119	
Formato: 	application/pdf	
Fonte: 	Aquatic Living Resources (0990-7440) (Edp Sciences S A), 2013-07 , Vol. 26 , N. 3 , P. 207-213	
Fonte: 	EDP Sciences, IFREMER, IRD 2013

info:eu-repo/semantics/openAccess