Data/hora: 02/06/2024 05:01:50

Provedor de dados: | 5
País: | France
Título: | Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment
Autores: | Morvezen, Romain; Cornette, Florence; Charrier, Gregory; Guinand, Bruno; Lapegue, Sylvie; Boudry, Pierre; Laroche, Jean.
Data: | 2013-07
Ano: | 2013
Palavras-chave: | Microsatellites; Polymerase chain reaction; Parentage; Assignment; Bivalve mollusc.
Resumo: | We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00137/24822/22907.pdf

DOI:10.1051/alr/2013052

Editor: | Edp Sciences S A

Relação:

http://archimer.ifremer.fr/doc/00137/24822/

info:eu-repo/grantAgreement/EC/FP7/245119

Formato: | application/pdf
Fonte: | Aquatic Living Resources (0990-7440) (Edp Sciences S A), 2013-07 , Vol. 26 , N. 3 , P. 207-213

Fonte:

EDP Sciences, IFREMER, IRD 2013

info:eu-repo/semantics/openAccess