Data/hora: 09/06/2024 09:24:21

Provedor de dados: | 5
País: | France
Título: | Expression of immune-related genes in the oyster Crassostrea gigas during ontogenesis
Autores: | Tirape, Ana; Bacque, Coralie; Brizard, Raphael; Vandenbulcke, Franck; Boulo, Viviane.
Data: | 2007-07
Ano: | 2007
Palavras-chave: | Rel/NF kappa B pathway; Cell proliferation; Hematopoiesis; Mollusk immunity; Hemocytes; Bivalve mollusk.
Resumo: | The work presented here reports the expression of immune-related genes during ontogenesis in the oyster Crassostrea gigar. Expression patterns of 18 selected genes showed that RNAs detected in oocytes and 2-4 cell embryos are of maternal origin and that gene transcription starts early after fertilization. The expression patterns of 4 genes (Cg-timp, Cg-tal, Cg-EcSOD and Drac3) suggested that hemocytes appear in the gastrula-trochophore stages. The localization of Cg-tal expression suggested that hematopoietic cells were derived from vessels and/or artery endothelia cells. Moreover, a bacterial challenge affected the level of expression of genes. Indeed, a change in expression levels was observed for Cg-LBP/BPI, Cg-timp, Drac3 and Cg-MyD88 genes in larval stages upon exposure to non-pathogenic bacteria. In early juveniles, a modulation was also observed for Cg-LBP/BPI, Cg-timp, Cg-MyD88 and for Cg-tal, according to the concentration of bacteria. Altogether, the results showed that studying the appearance of immunocompetent cells through their ability to express immune-related genes is a tool to gain insight the ontogenesis of the oyster immune system. (C) 2007 Elsevier Ltd. All rights reserved.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2007/publication-2612.pdf

DOI:10.1016/j.dci.2007.01.005

Editor: | Elsevier
Relação: | http://archimer.ifremer.fr/doc/00000/2612/
Formato: | application/pdf
Fonte: | Developmental &amp; Comparative Immunology (0145-305X) (Elsevier), 2007-07 , Vol. 31 , N. 9 , P. 859-873
Fonte: | 2007 Elsevier Ltd All rights reserved.