Data/hora: 11/06/2024 02:35:09

Provedor de dados: | Institutional Archive of Ifreme (ArchiMer)
País: | France
Título: | Adsorption of norovirus and ostreid herpesvirus type 1 to polymer membranes for the development of passive samplers
Autores: | Hubert, Francoise; Morga, Benjamin; Renault, Tristan; Le Guyader, Soizick.
Data: | 2017-04
Ano: | 2017
Palavras-chave: | Norovirus; OsHV-1; Pacific oysters; Passive samplers; Seawater; Sewage.
Resumo: | Aims  This study was performed to develop passive sampling methodology for the detection of two viruses in seawater in the area of shellfish production, The Norovirus (NoV), a human pathogen implicated in gastroenteritis outbreaks linked to oyster consumption and the ostreid herpesvirus type 1 (OsHV-1) a virus associated with mass mortalities of Pacific oysters. Methods and Results  Commercially membranes were tested for their capacity to adsorb virus: Zetapor, gauze, nylon, low density polyethylene (LDPE) and Polyvinylidene difluoride (PVDF). Laboratory exposures of membranes to contaminated water samples (stool, sewage, seawater) were performed. Our data shown that the amount of NoV GII genome per membrane measured with qRT-PCR increased with the time of exposure up to 24h, for all types of membranes except gauze. After 15 days of exposure, the amount of NoV GII per membrane continued to increase only for nylon and LDPE. The amount of OsHV-1 per zetapor membrane was significantly increased as soon as 4h of exposure, and after 24 h of exposure for all types of membranes. Exposure of membranes to serial dilutions of various samples revealed that the amount of NoV GII and OsHV-1 per membrane is significantly higher in diluted samples. The detection of NoV and OsHV-1 respectively with zetapor and PVDF membranes were found to be more efficient than direct analysis of sewage and sea water Conclusions: All membranes immerged in contaminated samples adsorbed NoV GII and OsHV-1. The amount of both virus increased with the time of exposure. Zetapor and PVDF membranes seems to be more adapted to NoV GII and OsHV-1 detection respectively. Significance and Impact of the study  Membranes tested will be used as passive samplers to improve the detection of virus in oyster production areas. Also, passive samplers could be a valuable tool for microbiome analysis with New Generation Sequencing.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00365/47646/47670.pdf

DOI:10.1111/jam.13394

http://archimer.ifremer.fr/doc/00365/47646/

Editor: | Wiley
Relação: | info:eu-repo/grantAgreement/EC/FP7/678589/EU//VIVALDI
Formato: | application/pdf
Fonte: | Journal Of Applied Microbiology (1364-5072) (Wiley), 2017-04 , Vol. 122 , N. 4 , P. 1039-1047

Fonte:

2017 The Society for Applied Microbiology

info:eu-repo/semantics/openAccess

restricted use