Data/hora: 08/06/2024 17:52:06

Provedor de dados: | 5
País: | France
Título: | Zeaxanthin from Porphyridium purpureum induces apoptosis in human melanoma cells expressing the oncogenic BRAF V600E mutation and sensitizes them to the BRAF inhibitor vemurafenib
Autores: | Juin, Camille; De Oliveira Junior, Raimundo Gonsalves; Fleury, Audrey; Oudinet, Chloe; Pytowski, Lior; Berard, Jean-baptiste; Nicolau, Elodie; Thiery, Valerie; Lanneluc, Isabelle; Beaugeard, Laureen; Prunier, Gregoire; Guedes Da Silva Almeida, Jackson Roberto; Picot, Laurent.
Data: | 2018-07
Ano: | 2018
Palavras-chave: | Cancer; Carotenoid; Melanoma; Microalgae; Phytoplankton; Zeaxanthin.
Resumo: | Zeaxanthin, an abundant carotenoid present in fruits, vegetables and algae was reported to exert antiproliferative activity and induce apoptosis in human uveal melanoma cells. It also inhibited uveal melanoma tumor growth and cell migration in nude mice xenograft models. Here we report that zeaxanthin purified from the rhodophyte Porphyridium purpureum (Bory) K.M.Drew &amp; R.Ross, Porphyridiaceae, promotes apoptosis in the A2058 human melanoma cell line expressing the oncogenic BRAF V600E mutation. Zeaxanthin 40 μM (IC50) induced chromatin condensation, nuclear blebbing, hypodiploidy, accumulation of cells in sub-G1 phase, DNA internucleosomal fragmentation and activation of caspase-3. Western blot analysis revealed that zeaxanthin induced up-regulation of the pro-apoptotic factors Bim and Bid and inhibition of NF-κB transactivation. Additionally, zeaxanthin sensitized A2058 melanoma cells in vitro to the cytotoxic activity of vemurafenib, a BRAF inhibitor widely used for the clinical management of melanoma, suggesting its potential interest as dietary adjuvant increasing melanoma cells sensitivity to chemotherapy.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00444/55595/57227.pdf

DOI:10.1016/j.bjp.2018.05.009

http://archimer.ifremer.fr/doc/00444/55595/

Editor: | Soc Brasileira Farmacognosia
Formato: | application/pdf
Fonte: | Revista Brasileira De Farmacognosia-brazilian Journal Of Pharmacognosy (0102-695X) (Soc Brasileira Farmacognosia), 2018-07 , Vol. 28 , N. 4 , P. 457-467

Fonte:

2018 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda. This is an openaccess article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

info:eu-repo/semantics/openAccess

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