Data/hora: 01/06/2024 17:34:19

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Experimental Design Applied to the Optimization and Partial Characterization of Pectin Liase from a Newly Isolated Penicillium brasilianum	
Autores: 	Zeni,Jamile; Gomes,Jonaína; Ambroszini,Éllin; Basso,Ana Paula; Toniazzo,Geciane; Valduga,Eunice.	
Data: 	2014-12-01	
Ano: 	2014	
Palavras-chave: 	Penicillium brasilianum; Pectin liase; Experimental design technique.	
Resumo: 	Penicillium brasilianum was previously isolated from tea and identified by molecular biology technique. A Plackett-Burman design, followed by a complete second order design was used for the screening of most important factors and to maximize the pectin liase (PMGL) activity, respectively. The maximum PMGL activity by P. brasilianum achieved was 9.0 U/mL after 48 h of cultivation in a medium containing pectin (33.0 g/L), yeast extract (30.0 g/L) and potassium phosphate (2.0 g/L) at 30ºC, with a stirring rate of 180 rpm, initial pH 5.5 and 5x106spores/mL inoculum size. The kinetic evaluation in terms of substrate consumption demonstrated that the maximum production of PMGL was at 72 h, and 40% of the total organic carbon, 25% of the nitrogen, 88% of the magnesium, 13% of the potassium and 66% of the iron were consumed. The pH remained almost stable during the whole period of production (5.33 to 4.9). The partial characterization of the crude PMGL enzyme extract showed optimal pH and temperature of 5.5 and 37°C, respectively.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600908	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-8913201402536	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.57 n.6 2014	
Fonte: 	info:eu-repo/semantics/openAccess