Data/hora: 09/06/2024 15:52:38

Provedor de dados: 	Brazilian Archives of Biology and Technology	
País: 	Brazil	
Título: 	Determination of nuclear transcription factor activity using a modified electrophoretic mobility shift assay	
Autores: 	Wang,Yong; Huang,Wenhua; Peng,Daizhi; Hu,Yuanbin.	
Data: 	2008-08-01	
Ano: 	2008	
Palavras-chave: 	Nuclear transcription factor; Electrophoretic mobility shift assay; Methodology; Modification.	
Resumo: 	In this work, several major procedures of the electrophoretic mobility shift assay (EMSA) were modified including swift extraction of the nucleic protein, labeling of the probe and radioautography. The modified assay required shorter time, simplified the nucleic protein extraction, increased the radioactivity of the labeling probe, skipped the tedious process of gel drying, and produced clear images. Its results were comparable, reproducible and stable. It thus has merited for wide application.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400025	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132008000400025	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.51 n.4 2008	
Fonte: 	info:eu-repo/semantics/openAccess