Data/hora: 02/06/2024 04:54:28

Provedor de dados: | Brazilian Archives of Biology and Technology
País: | Brazil
Título: | Improved purification process of β- and α-trypsin isoforms by ion-exchange chromatography
Autores: | Santos,Alexandre Martins Costa; Oliveira,Jamil Silvano de; Bittar,Eustáquio Resende; Silva,Anderson Lourenço da; Guia,Marcos Luiz dos Mares; Bemquerer,Marcelo Porto; Santoro,Marcelo Matos.
Data: | 2008-08-01
Ano: | 2008
Palavras-chave: | Trypsin isoforms; Resolution; Ion-exchange chromatography; Purification; Specific activity; Mass spectrometry.
Resumo: | The purpose of this work was to improve the separation and yield of pure β- and α-trypsin isoforms by ion-exchange chromatography and to characterize some physical-chemical properties of these isoforms. Purification of trypsin isoforms was performed by ion-exchange chromatography in 0.1 mol/L tris-HC buffer, pH 7.10 at 4ºC. The sample loading, salt concentration, flow rate and pH of mobile phase were varied to determine their effects on the resolution of the separation. The resolution was optimized mainly between β- and α-trypsin. Pure isoforms were obtained by chromatographying 100 mg of commercial trypsin during seven days, yielding 51 mg of high purity β-trypsin and 13 mg of α-trypsin partially pure, with small amounts of contaminating of ψ-trypsin. Thus, time and resolution of purification were optimized yielding large amounts of pure active enzymes that are useful for several research areas and biotechnology.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400009
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132008000400009
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.51 n.4 2008
Fonte: | info:eu-repo/semantics/openAccess