Data/hora: 09/06/2024 03:27:26

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Biochemical and morphological responses to abiotc elicitor chitin in suspension-cultured sugarcane cells	
Autores: 	Gallão,Maria Izabel; Cortelazzo,Ângelo Luiz; Fevereiro,Manuel Pedro Salema; Brito,Edy Sousa de.	
Data: 	2010-04-01	
Ano: 	2010	
Palavras-chave: 	Chitin; Elicitation; Peroxidase; PAL; Sugarcane; Tissue culture.	
Resumo: 	Cells of Saccharum officinarum submitted to hydrolyzated chitin for 1 to 8h produced phenolic compounds. These alterations were observed through cytochemical methods using Toluidine Blue and Phloroglucinol/HCl. After 4 h, besides cell wall change, there was a change in nuclear pattern of chitin treated cells. There was a 96% increase in nuclear area in 6 h chitin treated material, as observed by Feulgen reaction. The treated cells showed chromatin compacted regions and a degeneration process of nucleoli. In the outer areas of cell wall, there was a polysaccharide desagregation, confirming results obtained for different plants with the use of other elicitors. Peroxidase activity was maximal after 4 h and decreased progressively. PAL activity started to increase at 4 h of incubation. These results showed that chitin hydrolyzate stimulated a defense response in sugarcane cells.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200002	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132010000200002	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.53 n.2 2010	
Fonte: 	info:eu-repo/semantics/openAccess