Data/hora: 03/06/2024 04:39:48

Provedor de dados: | Brazilian Archives of Biology and Technology
País: | Brazil
Título: | Plant or fungal sequences? An alternative optimized PCR protocol to avoid ITS (nrDNA) misamplification
Autores: | Miranda,Vitor Fernandes Oliveira de; Martins,Vanderlei Geraldo; Furlan,Antonio; Bacci Jr.,Maurício.
Data: | 2010-02-01
Ano: | 2010
Palavras-chave: | Polymerase; DNA; Drosera; Fungi; Phylogeny.
Resumo: | The nuclear ribosomal DNA internal transcribed spacers (ITS1 and ITS2) from leaves of Drosera (Droseraceae) were amplified using "universal" primers. The analysis of the products demonstrated most samples were a molecular mixture as a result of unsuccessful and non-specific amplifications. Among the obtained sequences, two were from Basidiomycota fungi. Homologous sequences of Basidiomycota were obtained from GenBank database and added to a data set with sequences from Drosera leaves. Parsimony analysis demonstrated that one sequence was amplified from an Ustilaginomycetes fungus, and another from a Heterobasidiomycetes. Possibly these fungi were associated to leaves of Drosera, and not because of samples contamination. In order to provide optimization and a better specificity of PCR (polymerase chain reaction), a very successful method was demonstrated using dimethyl sulfoxide (DMSO) and bovine serum albumin (BSA) in reactions.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100018
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132010000100018
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.53 n.1 2010
Fonte: | info:eu-repo/semantics/openAccess