Data/hora: 16/06/2024 11:07:28

Provedor de dados: | 52
País: | Brazil
Título: | Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains
Autores: | Oliveira,Arlem Nascimento de; Oliveira,Luiz Antonio de; Andrade,Jerusa Souza.
Data: | 2010-10-01
Ano: | 2010
Palavras-chave: | Rhizobium; Bradyrhizobium; Enzymatic activities; Organic surfactants.
Resumo: | Two rhizobia strains isolated from soils of the Central Amazonian floodplain produced appreciable quantities of crude alkaline protease extracts with inexpensive carbon and nitrogen sources. These protease crude extracts were optimally active at pH 9.0-11.0. The optimum temperatures were 35 ºC for Rhizobium sp. strain R-986 and 55 ºC for Bradyrhizobium sp. strain R-993. Protease activities in the crude extracts were enhanced in the presence of 5 mM metal ions, such as Na+, Ca2+, Mg2+ and Mn2+. Rhizobia proteases were strongly inhibited by PMSF, a serine-protease inhibitor. The enzymes were active in the presence of surfactants (SDS and Triton X-100) and stable in oxidizing (H2O2) and reducing agents (β-mercaptoethanol), and organic solvents (acetone, hexane, methanol, 1-propanol and toluene).
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500024
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132010000500024
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.53 n.5 2010
Fonte: | info:eu-repo/semantics/openAccess