Data/hora: 10/06/2024 12:21:06

Provedor de dados: | 52
País: | Brazil
Título: | A comparison of methods to determine tannin acyl hydrolase activity
Autores: | Aguilar,Cristóbal; Christopher,Augur; Viniegra-González,Gustavo; Favela,Ernesto.
Data: | 1999-01-01
Ano: | 1999
Palavras-chave: | Tannin acyl hydrolase; Assay; Polyurethane foam; Solid state fermentation; Tannase.
Resumo: | Six methods to determine the activity of tannase produced by Aspergillus niger Aa-20 on polyurethane foam by solid state fermentation, which included two titrimetric techniques, three spectrophotometric methods and one HPLC assay were tested and compared. All methods assayed enabled the measurement of extracellular tannase activity. However, only five were useful to evaluate intracellular tannase activity. Studies on the effect of pH on tannase extraction demonstrated that tannase activity was considerably under-estimated when its extraction was carried out at pH values below 5.5 and above 6.0. Results showed that the HPLC technique and the modified Bajpai and Patil methods presented several advantages in comparison to the other methods tested.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300014
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89131999000300014
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.42 n.3 1999
Fonte: | info:eu-repo/semantics/openAccess