Data/hora: 10/06/2024 17:22:56

Provedor de dados: 	52	
País: 	Brazil	
Título: 	A comparison of methods to determine tannin acyl hydrolase activity	
Autores: 	Aguilar,Cristóbal; Christopher,Augur; Viniegra-González,Gustavo; Favela,Ernesto.	
Data: 	1999-01-01	
Ano: 	1999	
Palavras-chave: 	Tannin acyl hydrolase; Assay; Polyurethane foam; Solid state fermentation; Tannase.	
Resumo: 	Six methods to determine the activity of tannase produced by Aspergillus niger Aa-20 on polyurethane foam by solid state fermentation, which included two titrimetric techniques, three spectrophotometric methods and one HPLC assay were tested and compared. All methods assayed enabled the measurement of extracellular tannase activity. However, only five were useful to evaluate intracellular tannase activity. Studies on the effect of pH on tannase extraction demonstrated that tannase activity was considerably under-estimated when its extraction was carried out at pH values below 5.5 and above 6.0. Results showed that the HPLC technique and the modified Bajpai and Patil methods presented several advantages in comparison to the other methods tested.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300014	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89131999000300014	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.42 n.3 1999	
Fonte: 	info:eu-repo/semantics/openAccess